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KMID : 0384119960160030269
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Korean Journal of Clinical Pathology
1996 Volume.16 No. 3 p.269 ~ p.279
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Bcl-2 Oncoprotein Expression and bcl-2/JH Gene Rearrangement in Lymphoproliferative Disease
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Abstract
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Background:
@EN Bcl-2 gene product, bcl-2 protein, is an intracellular protein found primarily in inner mitochondrial membrane and plays a major role to lock apoptosis. The bcl-2 expression is up-regulated by t(14; 18) chromosomal translocation that is
frequently
observed in B-cell non-Hodgkin's lymphoma (NHL). The purpose of this study is to investigate the frequency of bcl-2 expression in lymphoproliferative diseases and to define the relationship between bcl-2 expression and bcl-2/JH gene
rearrangement.
@ES Methods:
@EN Fifty-two lymphoproliferative diseases involving bone marrow were evaluated for bcl-2 expression and bcl-2JH gene rearrangement. Immunohistochemical staining for bcl-2 protein was performed on formalin-fixed, paraffin-embedded marrow sections
by
using anti-human bcl-2 protein mouse monoclonal antibody(Dako, bcl-2 124, USA). DNA were extracted from the stored bone marrow slides and paraffin-embedded biopsy specimens. Nested polymerase chain reaction(PCR) was applied for the amplification
of
bcl-2/JH fusion gene.
@ES Results:
@EN Of the 52 cases, 30 showed bcl-2 protein expression and 12 revealed bcl2/JH gene rearrangement, All the 12 cases, who showed bcl-2/JH gene rearrangement, were positive for bcl-2 protein immuostaining Of the 45 HNL cases, 16 revealed bcl-2
protein
expression, however, no apparent gene rearrangements were noted in these cases. Among 6 chronic lymphocytic leukemia (CLL), 2 cases who showed no bcl-2/JH gene rearrangements, were positive for bcl-2 protein immunostaining.
@ES Conclusions:
@EN Bcl-2 protein expression appears to be up-regulated by bcl-2/JH gene rearrangement, however, a substantial proportion(35%) of lymphoproliferative diseases seems to express high levels of bcl-2 protein without relation to gene rearrangement.
These
data suggest that another mechanisms to activate protein expression than bcl-2/JH rearrangement are present in lymphoproliferative diseases (Korean J Clin Pathol 1996;16(3):269~79).
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